Part:BBa_K3225014:Design

J23101-LuxR-pLux-GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1037
  

Design Notes

We built this device with Gibson assembly, therefore there is no biobrick scar between two parts.

Source

Sequence of LuxR and pLux is from part BBa_C0062 and BBa_R0062.The physical samples of them is de-novo synthesis.